12th Class Biology Chapter 23 MCQs with Answers

Welcome to the 12th Class Biology Chapter 23 MCQs Online Test. We are presenting you with top MCQ questions from the 12th Class Biology Chapter Biotechnology.

We want to tell you that you can find all the 12th Class Biology Chapter 23 MCQs online tests on our website. These online tests are great for learning and as well as for scoring maximum marks in your Intermediate Exams. We are making these Class 12 Biology MCQs online tests for those who want full marks in their exams. Attempt all these Grade 12 Biology Online Multiple Choice Questions MCQs before going for the exam and it will be beneficial for you! These online tests contain all the important multiple choice questions in the important topics of the FSC Part 2 Biology Chapter 23 Biotechnology 

12th Class Biology Chapter 23 MCQs with Answers

Youth For Pakistan has provided an excellent opportunity for the students of the 12th class to prepare their Biology Chapter 23 Biotechnology online with MCQs test.

This is a free platform for students to prepare for Biology Exams for Class 12 to get the best accomplishments in annual exams. 12th class Students can analyze themself by attempting this online test system as many times as they want until they find themself fully prepared.

MCQs Class 12 Biology with Answers

The purpose of these online MCQs tests is to help you evaluate your 12th Class Biology Chapter 23 MCQs. These Multiple Choice Questions (MCQs) will prepare you for your academic success in the 12th Class Exams. 12th Class Biology Chapters include Ch#15 Homeostasis, Ch#16 Support and Movement, Ch#17 Coordination and Control, Ch#18 Reproduction, Ch#19 Growth and Development, Ch#20 Chromosome and DNA, Ch#21 Cell Cycle, Ch#22 Variation and Genetics, Ch#23 Biotechnology, Ch#24 Evolution, Ch#25 Ecosystem, Ch#26 Some major Ecosystems and Ch#27 Man and his Environment

What is Biotechnology?

Biotechnology is a broad discipline in which biological processes, organisms, cells or cellular components are exploited to develop new technologies. New tools and products developed by biotechnologists are useful in research, agriculture, industry, and the clinic.

12th Class Biology Chapter 23 MCQs: Biotechnology

1. Mendel’s work was rediscovered in choose which one is correct:

  • 1868
  • 1882
  • 1900
  • 1950

2. The work of biotechnology is choose which one is correct:

  • Insulin
  • Drugs
  • Pollution
  • All of the above

3. Production of identical copying of gene is choose which one is correct:

  • Replication
  • Cloning
  • Reproduction
  • Multiplication

4. Which of the following techniques produces large number of copies of genes?

  • PCR
  • Recombinant DNA technology
  • RFLP
  • None of the above

5. The technique used to produce copies of genes in laboratory is choose which one is correct:

  • PCR
  • Recombinant DNA technology
  • RFLOP
  • None of the above

6. Which of the followings is used to transfer genes?

  • Molecular scissors
  • Molecular vector
  • Expression system
  • None of the above

7. The system used for the formation of specific product is choose which one is correct:

  • Molecular scissors
  • Molecular vector
  • Expression system
  • Gene of interest

8. Genes are cut by choose which one is correct:

  • Gene of interest
  • Expression system
  • Molecular vector
  • Endonucleases

9. The genes which are synthesized from mRNA by reverse transcriptase are

called choose which one is correct:

  • Endonucleases
  • Plasmids
  • Coda
  • None of the above

10. Restriction enzymes are present in choose which one is correct:

  • Virus
  • Bacteria
  • Plasmids
  • All of the above

11. The first restriction enzyme was isolated by choose which one is correct:

  • Kary Mullis
  • Hamiton
  • Sanger
  • Maxam

12. Palindromic sequences are present in choose which one is correct:

  • Single phase
  • Repeated form
  • Reverse order
  • Similar order

13. Number of restriction enzymes discovered so far are choose which one is correct:

  • 200
  • 300
  • 400
  • 500

14. The number of frequently used restriction enzymes are choose which one is correct:

  • 10
  • 20
  • 30
  • 40

15. The commonly used restriction enzyme is choose which one is correct:

  • E.Coli
  • EcoR11
  • EcoR1
  • EcoR111

16. Natural extra chromosomal circular DNA are choose which one is correct:

  • Endonucleases
  • Restriction enzyme
  • Plasmids
  • Phage virus

17. Which of the followings is not a vector?

  • pSc 101
  • pBR-322
  • EcoR1
  • Phage virus

18. The plasmids having resistant gene against tetracycline and ampicillin are choose which one is correct:

  • pSc 101
  • pBR-322
  • EcoR1
  • Phage virus

19. Which of the following phages is use as vector?

  • Bacteriophage
  • Lambda phage
  • T phage
  • All of the above

20. Pieces of DNA are jointed by choose which one is correct:

  • Chimaeric DNA
  • DA ligase
  • Plasmids
  • Endonucleases

21. Match recombinant DNA with one of the followings choose which one is correct:

  • Chimaeric DNA
  • DNA ligase
  • Plasmids
  • Endonucleases

22. Recombinant DNA are expressed in choose which one is correct:

  • Man
  • Virus
  • Bacteria
  • None of the above

23. The chemical that makes the bacterial membrane permeable is choose which one is correct:

  • NaCl
  • CaCl2
  • Na2CO3
  • CaCO3

24. The single standard nucleotide sequence that hybridize into certain piece is choose which one is correct:

  • Clone
  • Probe
  • REFLP
  • Endonuclease

25. Which of the followings form genomic library?

  • Bacteria
  • Bacteriophage
  • Segment of DNA
  • None of the above

26. PCR technique was developed by choose which one is correct:

  • Kary Mullis
  • Hamilton
  • Sanger
  • Maxam

27. Which of the followings is irrelevant for PCR?

  • Primer DNA
  • Recombinant DNA
  • DNA polymerase
  • Thermocycler

28. Match target DNA with one of the followings choose which one is correct:

  • Primer DNA
  • Recombinant DNA
  • DNA polymerase
  • Thermocycler

29. The enzyme extracted from Thermus aquaticus is choose which one is correct:

  • Primer DNA
  • Recombinant DNA
  • DNA polymerase
  • Thermocycler

30. The steps involved in DNA finger printing choose which one is correct:

  • RFLP
  • Probes
  • PCR
  • All of the above

31. The collection of different sized fragments is choose which one is correct:

  • Probes
  • RFLP
  • PCR
  • Gel

32. DNA analyzer is used for choose which one is correct:

  • Forensic
  • Diagnosis
  • Evolution
  • All of the above

33. The use of DNA analysis for convicting the criminal of rape is used in choose which one is correct:

  • Diagnosis
  • Forensic
  • Evolution
  • All of the above

34. Match Sanger’s method for generation of pieces of DNA with one of the

followings choose which one is correct:

  • Deoxyribonucleotide
  • Ribonucleoside
  • Dideoxyribonucleoside
  • DNA threads

35. Match Maxam Gilbert method for generation of pieces of DNA with one of

the followings choose which one is correct:

  • Deoxyribonucleotide
  • Dexoyribonucleoside
  • Dideoxyribonucleoside
  • DMA threads

36. Pieces of DNA are separated on choose which one is correct:

  • Agarol
  • Agarose gel
  • Agar
  • All of the above

37. In gene sequencing the nucleotide strained with fluorescent dye are choose which one is correct:

  • Initiation nucleotides
  • Middle nucleotides
  • Terminating nucleotides
  • None of the above

38. In gene sequencing, the colour of the fluorescent bands are read by choose which one is correct:

  • Computer
  • Gel
  • Detector
  • Laser beam

39. The nucleotide sequence is stored by choose which one is correct:

  • Computer
  • Gel
  • Detector
  • Laser beam

40. The DNA sequence of which of the followings have been done?

  • Chloroplast
  • Mitochondria
  • Yeast
  • All of the above

41. Which of the followings is model plant whose gene sequencing has been completed?

  • Yeast
  • Pea
  • Arabidopsis
  • Plum

42. The gene sequence of which of the following chromosomes was completed in

1999 choose which one is correct:

  • 12
  • 18
  • 21
  • 22

43. The smallest human chromosomes is choose which one is correct:

  • 10
  • 22
  • 23
  • Y

44. Which of the followings helped scientist to pinpoint disease causing genes?

  • PCR
  • Recombinant DNA
  • RFLP
  • Probe

45. The number of base sequences in man is choose which one is correct:

  • 2 billion
  • 3 billion
  • 4 billion
  • 5 billion

46. The human genomic project will take how many volume of encyclopedia choose which one is correct:

  • 100
  • 200
  • 300
  • 400

47. Each piece of DNA should be choose which one is correct:

  • 500 nucleotide
  • 1000 nucleotide
  • 3000 nucleotide
  • 4000 nucleotide

48. The copy of DNA pieces in human genomic project is made by choose which one is correct:

  • PCR
  • Recombinant DNA
  • RFLP
  • Probe

49. The entire genome has been sequence by the company of choose which one is correct:

  • J. Craig Venter
  • Hamilton
  • Sanger
  • Maxam

50. Organisms with foreign genes are called choose which one is correct:

  • Recombinant
  • Hybrids
  • Transgenic
  • Modified

51. Transgenic bacteria are produced in large vats called choose which one is correct:

  • Thermocycler
  • Bioreactors
  • Reactors
  • Electrophoresis

52. Which of the following products is prepared by recombinant DNA technology?

  • Hepatitis B vaccine
  • Growth hormone
  • Insulin
  • All of the above

53. The degradation of pollutants with the help of recombinant bacteria is called choose which one is correct:

  • Biofilters
  • Biodegradation
  • Bioabsorption
  • None of the above

54. The bacteria used in industries for control of pollution are choose which one is correct:

  • Bioabsorption
  • Biodegradation
  • Biofilters
  • None of the above

55. The genes which cause self destruction of bacteria are choose which one is correct:

  • Phage genes
  • Suicide genes
  • Recombinant genes
  • Plasmid genes

56. Naturasweet is choose which one is correct:

  • Sugar
  • Starch
  • Aspartame
  • Glucose

57. The bacteria are used in mining industry for choose which one is correct:

  • Bioabsorption
  • Biodegradation
  • Biofilters
  • Bioleaching

58. The plant cells whose cell wall is removed are choose which one is correct:

  • Naked cells
  • Cullous
  • Protoplast
  • All of the above

59. In which of the followings plant pest and herb resistant genes are not used?

  • Cotton
  • Corm
  • Yeast
  • Potato

60. The acreage of transgenic plants in 1999 was choose which one is correct:

  • 50 million
  • 70 million
  • 90 million
  • 100 million

61. Green revolution was launched in choose which one is correct:

  • 1960
  • 1970
  • 1990
  • 2000

62. Scientists are working on which of followings for increasing yield choose which one is correct:

  • Stomata
  • RuBP carboxylase
  • C4 cycle
  • All of the above

63. Biodegradable plastic is obtained from choose which one is correct:

  • Yeast
  • Mouse eared cress
  • Bacteria
  • Bioreactos

64. The antibody which can deliver radio isotopes to tumor cells is obtained

from choose which one is correct:

  • Yeast
  • Weed
  • Corn
  • Wheat

65. The antibody which is used for the treatment of genital herps is obtained

from choose which one is correct:

  • Yeast
  • Soya bean
  • Corn
  • Wheat

66. Gene pharming is used for obtaining choose which one is correct:

  • Milk
  • Meat
  • Antibodies
  • Drugs

67. In gene pharming egg is fertilized choose which one is correct:

  • In vitro
  • In vivo
  • In bioreactor
  • None of the above

68. Antithrombin is produced in choose which one is correct:

  • Sheep
  • Goat
  • Mice
  • Bovine

69. The organisms which are used to produce human growth hormone in their

urine are choose which one is correct:

  • Sheep
  • Goat
  • Mice
  • Bovine

70. The production of genetically identical copies of the organisms by asexual

reproduction is called choose which one is correct:

  • Recombinant DNA
  • Cloning
  • Gene pharming
  • None of the above

71. The first animal cloned was choose which one is correct:

  • Sheep
  • Dolly
  • Mice
  • Bovine

72. In the cloning of mice the second nucleus is obtained from choose which one is correct:

  • Uterus cells
  • Cumulus cells
  • Skin cells
  • None of the above

73. The insertion of genetic material into human cells for the treatment of a

disorder is called

  • Gene cloning
  • Gene pharming
  • Gene therapy
  • Gene sequencing

74. The disease not treated by Ex vivo gene therapy is choose which one is correct:

  • SCID
  • Diabetes
  • Hypercholesterolemia
  • All of the above

75. The enzyme ADA is involved in maturation of choose which one is correct:

  • RBC
  • Platelets
  • Lymphocyte
  • Macrophages

76. The organism used as vector during treatment of SCID by gene therapy is choose which one is correct:

  • Plasmids
  • Phage
  • Retroviruses
  • None of the above

77. The genes for the treatment of SCID are introduced in choose which one is correct:

  • Bone marrow
  • Heart
  • Liver
  • None of the above

78. The hypercholesterolemia causes at young age choose which one is correct:

  • Diabetes
  • Blood pressure
  • Heart attack
  • All of the above

79. The genes for the treatment of hypercholesterolemia are introduced in choose which one is correct:

  • Bone marrow
  • Heart
  • Liver
  • None of the above

80. In which of these in vivo gene therapy is used?

  • Cystic fibrosis
  • Cancer
  • Coronary angioplasty
  • All of the above

81. In cystic fibrosis, the gene codes for trans membrane carrier of which ion is

missing choose which one is correct:

  • Na
  • K
  • Cl
  • Ca

82. The microscopic vesicles formed during gene therapy of cystic fibrosis are

called choose which one is correct:

  • Ribosomes
  • Lipososmes
  • Endosomes
  • Microsomes

83. The solution of Liposomes is sprayed on choose which one is correct:

  • Skin
  • Joints
  • Nostrils
  • Mouth

84. The gene therapy in cancer makes the patient tolerant for choose which one is correct:

  • Radiotherapy
  • Chemotherapy
  • Endoscopies
  • Gene therapy

85. These are used in angiosplasty choose which one is correct:

  • Balloon
  • Plasmids
  • Gene therapy
  • All of the above

86. Which of the following diseases is not treated by in vivo gene therapy?

  • Haemophilia
  • Diabetes
  • Parkinson’s diseases
  • All of the above

87. The plants cells can develop complete plants. So they are called choose which one is correct:

  • Callous
  • Totipotent
  • Clone
  • None of the above

88. Complete carrot plant was developed from tiny part of phloem by choose which one is correct:

  • J. Craig Venter
  • Hamilton
  • Sanger
  • F.C. Steward

89. F. C Steward provided the phloem cells choose which one is correct:

  • Sugars
  • Vitamins
  • Coconut milk
  • All of the above

90. An undifferentiated group of cells is called choose which one is correct:

  • Callous
  • Totipotent
  • Clone
  • None of the above

91. Callous can form choose which one is correct:

  • Root
  • Stem
  • Shoot
  • Complete plant

92. In micropropagation, the structure used is choose which one is correct:

  • Stem
  • Meristem
  • Callous
  • Totipotent

93. In micropropagation of meristem, the hormone used is choose which one is correct:

  • Gibberellins
  • Auxins
  • Abscisic acid
  • All of the above

94. The plants produced by micropropagation of meristem are called choose which one is correct:

  • Callous
  • Seedling
  • Clonal
  • Somaclonal

95. Which of the following is virus free?

  • Callous
  • Seedling
  • Clonal plant
  • Adult plant

96. The naked cells of plant are called choose which one is correct:

  • Protoplasm
  • Cytoplasm
  • Protoplast
  • Callous

97. The protoplast regenerates and produces choose which one is correct:

  • Callous
  • Seedling
  • Clonal
  • Clump

98. The clumps produce choose which one is correct:

  • Callous
  • Somatic embryo
  • Clonal plant
  • Adult plant

99. Somatic embryo are encapsulated in gel and are called choose which one is correct:

  • Seeds
  • Artificial seed
  • Clonal plant
  • Clump

100. Millions of artificial seed are produced in large tanks called choose which one is correct:

  • Thermocycler
  • Biopole
  • Bioreactors
  • None of the above

101. The artificial seeds are produced of which of the following plants?

  • Corn
  • Tomato
  • Mango
  • Roses

102. The plants produced by micropropagation from single cell are called choose which one is correct:

  • Callous
  • Seedling
  • Clonal
  • Somaclonal

103. Anther is cultured in a medium containing choose which one is correct:

  • Sugars
  • Vitamins
  • Coconut milk
  • All of the above

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